We assayed the ion channel properties of a CFTR bearing a mutation in the fifth putative transmembrane segment, G314E, which has been associated with cystic fibrosis (Golla et al., Hum. Mutation 3:67, 1994). Mutant and wild-type CFTRs were expressed in Xenopus oocytes and macroscopic, cAMP-activated currents were measured using a two-electrode.
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